KMID : 1225720130050010048
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Allergy, Asthma & Immunology Research : AAIR 2013 Volume.5 No. 1 p.48 ~ p.54
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In Vitro Induction of Allergen-Specific Interleukin-10-Producing Regulatory B Cell Responses by Interferon-¥ã in Non-Immunoglobulin E-Mediated Milk Allergy
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Lee Soo-Jin
Noh Geun-Woong Lee Jae-Ho
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Abstract
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Purpose: Specific oral immunotherapy (SOIT) using interferon-¥ã (IFN-¥ã) has been successful as a food allergy treatment. Interleukin-10 (IL-10)-producing regulatory B cells (Br1s) play a role in immune tolerance to food allergens. In addition, IFN-¥ã shows tolerogenic effects on allergen-induced Br1 responses.
Methods: Eleven patients that were allergic to cow¡¯s milk and 12 milk-tolerant subjects were selected by double-blind placebo-controlled food challenge (DBPCFC) and clinical characteristics. The immunomodulatory effects of IFN-¥ã on allergen-specific Br1 responses were evaluated in 6 milk allergy patients and 8 milk-tolerant subjects. Peripheral blood mononuclear cells (PBMCs) from subjects were stimulated with casein and/or IFN-¥ã and analyzed by flow cytometry.
Results: IFN-¥ã had no effect on total cell counts or the proportion of Br1 cells in PBMCs. IFN-¥ã stimulation did not change total Br1 cell counts or the percentage of Br1s among CD5(+) B cells in the milk allergy or the milk-tolerant groups. In the milk allergy group, Br1 counts were not different between the control and the casein stimulation but significantly increased in the IFN-¥ã + casein stimulated cells, and the Br1 fractions were decreased after casein stimulation and recovered in the addition of IFN-¥ã for stimulation. In the milk-tolerant group, Br1 counts increased in the casein stimulated cells and in the IFN-¥ã + casein stimulated cells, but the increase was significantly less when IFN-¥ã was added, and the Br1 fractions were increased after casein stimulation and IFN-¥ã + casein stimulation, that was not significant when IFN-¥ã was added.
Conclusions: IFN-¥ã-induced allergen-specific Br1 responses in the PBMCs of milk allergy patients play a role in milk allergen-specific tolerance induction in vitro. Further investigations into the molecular immunological mechanisms underlying the induction of allergen-specific Br1 responses are needed.
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KEYWORD
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Atopic dermatitis, IFN-¥ã, IL-10-producing regulatory B cell, non-IgE-mediated milk allergy, oral immunotherapy, tolerance
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